Making Internal Amplification Control Mandatory for Diagnostic PCR
نویسندگان
چکیده
منابع مشابه
Making internal amplification control mandatory for diagnostic PCR.
The explosive increase since the beginning of 1990s in the number of publications reporting PCR-based methods for detection or molecular typing of food-borne pathogens has attracted the attention of end user laboratories. However, the well-recognized difficulties in reproducing published tests due to variation in the performance of PCR thermal cyclers (7) and in efficiencies of different DNA po...
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Various constituents in clinical specimens, particularly feces, can inhibit the PCR assay and lead to false-negative results. To ensure that negative results of a diagnostic PCR assay are true, it should be properly monitored by an inhibition control. In this study, a cloning vector harboring a modified target DNA sequence (≈375 bp) was constructed to be used as a competitive internal amplifica...
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Reverse transcription polymerase chain reaction (RT-PCR) is gaining major importance in the field of medical diagnostics. For example, RT-PCR assays are being tested to become standard methodology for detection of occult tumor cells in peripheral blood of cancer patients. However, there is heterogeneity in the frequency of tumor cell detection, e.g., in melanoma patients as reported in the lite...
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background pcr is a molecular technique for herpes simplex virus (hsv) detection that can cause life-threatening infections such as encephalitis and keratitis. however, the main issues, false-negative results causing by pcr inhibitors, of this technique that reduce pcr efficiency. to overcome this problem, a competitive internal amplification control (iac) was constructed for conventional pcr u...
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BACKGROUND PCR is a molecular technique for herpes simplex virus (HSV) detection that can cause life-threatening infections such as encephalitis and keratitis. However, the main issues, false-negative results causing by PCR inhibitors, of this technique that reduce PCR efficiency. To overcome this problem, a competitive internal amplification control (IAC) was constructed for conventional PCR u...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2003
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.41.12.5835.2003